《植物生理学报》 2014, 50(10): 1479-1493

高等植物醇脱氢酶及其基因家族研究进展

刘威¹, 陈昊¹, 靳亚忠^1,2, 齐红岩^1,*

¹沈阳农业大学园艺学院, 设施园艺省部共建教育部重点实验室, 辽宁省设施园艺重点实验室, 沈阳110866; ²黑龙江八一农垦大学农学院, 黑龙江大庆163319

通信作者：齐红岩；E-mail: hyqiaaa@126.com；Tel: 024-88342305

摘　要：

醇脱氢酶属于高等植物中普遍存在的一个锌结合脱氢/还原蛋白超家族, 根据作用底物不同, 将高等植物中的醇脱氢酶分为3个家族: 乙醇脱氢酶(alcohol dehydrogenase, ADH)、肉桂醇脱氢酶(cinnamyl alcohol dehydrogenase, CAD)、甲醛脱氢酶(formaldehyde dehydrogenase, FDH)。3个家族均不同程度地响应植物逆境胁迫, 不仅受低氧胁迫等逆境的诱导, 也受ABA等激素的调控。CAD催化木质素合成, 参与构建植物防御体系。ADH在植物香气物质合成中发挥作用, 受乙烯等激素调控, 选择性地进行短的直链醇和醛之间的相互转化, 催化香气物质前体的合成。本文综述了醇脱氢酶家族在高等植物中对逆境的响应、木质素和香气物质合成方面的研究概况, 以期为醇脱氢酶的深入研究提供参考。

关键词：乙醇脱氢酶; 肉桂醇脱氢酶; 甲醛脱氢酶; 逆境胁迫; 香气

收稿：2014-05-14   修定：2014-08-26

资助：辽宁省重大项目(2011215003)和辽宁省高等学校优秀人才支持计划(LR2014020)。

Advances in Alcohol Dehydrogenase Enzymes and Their Gene Families in Higher Plants

LIU Wei¹, CHEN Hao¹, JIN Ya-Zhong^1,2, QI Hong-Yan^1,*

¹Key Laboratory of Protected Horticulture of Liaoning Province, Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agriculture University, Shenyang 110866, China; ²College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Corresponding author: QI Hong-Yan; E-mail: hyqiaaa@126.com; Tel: 024-88342305

Abstract:

Alcohol dehydrogenases belong to a Zn-binding dehydrogenase/reductase protein superfamily commonly existing in higher plants, and which are classified into three families according to their substrate specialties: alcohol dehydrogenase (ADH), cinnamyl alcohol dehydrogenase (CAD), and formaldehyde dehydrogenase (FDH). All the three family members act differently in response to stresses, and they are not only response to the stresses such as oxygen deficiency, but also regulated by hormones such as ABA. CADs family members catalyze the synthesis of lignin and take part in the formation of defense. In addition, ADHs also act in the synthesis of aroma volatiles in which pathway they are regulated by hormones such as ethylene and inter-convert ethanol and acetaldehyde (and other short linear alcohol/aldehyde pairs) selectively to form the precursors of aroma volatiles. This paper reviews the research status of alcohol dehydrogenase superfamily in higher plants response to stresses, lignin and aroma volatiles synthesis, proposing to provide a reference for alcohol dehydrogenase further study.

Key words: alcohol dehydrogenase; cinnamyl alcohol dehydrogenase; formaldehyde dehydrogenase; stresses; aroma volatiles